Molecular cloning and functional characterization of a soybean GmGMP1 gene reveals its involvement in ascorbic acid biosynthesis and multiple abiotic stress tolerance in transgenic plants

摘要

cqvip:L-Ascorbic acid(As A) plays an important role in plants and animals. In plants, GDP-D-mannose pyrophosphorylase(GMP) is essential in the As A biosynthetic pathway. However, little is known about the genes encoding GMP in soybean and here we report genetic and functional analysis of the Gm GMP1(Glycine max GDP-D-mannose pyrophosphorylase 1) gene in this species. Gm GMP1 encoded a GDP-mannose pyrophosphorylase and exhibited higher transcript levels in the leaf than in the root, stem, flower, and seed. Transcript of this gene was ubiquitous in the vegetative and reproductive organs, and was induced by abiotic stress and light. Increasing expression of Gm GMP1 in Arabidopsis and soybean through an overexpressing approach caused pronounced enhancement of As A content, and was implicated in lowering the superoxide anion radical content and lipid peroxidation levels in Arabidopsis, and conferring tolerance to osmotic and high salt stresses during seed germination. The present study represents the first systematic determination of soybean genes encoding GDP-mannose pyrophosphorylase and provides useful evidence for the functional involvement of Gm GMP1 in control of As A content and conferring tolerance to osmotic and salt stress.