冠心病与MBL基因多态性及血浆MBL和hs-CRP水平的相关性

摘要

Objective To analyze the correlation between coronary heart disease(CHD) and the gene polymorphism of mannan binding lectin(MBL), the plasma contents of MBL and the level of high-sensitivity C-reactive protein (hs-CRP).Methods The anti-coagulated blood of CHD patients (group CHD, 100 cases) and healthy people(group C,60 cases) was collected.The point mutation of MBL ExonI 54 codon was detected by PCR-RFLP.The content of MBL and the level of hs-CRP were detected by ELISA kit and immunoturbidimetry, respectively.Results There was no significant difference in the genotypes of MBL ExonI 54 codon between group CHD and group C(P＞0.05).The plasma content of MBL and the level of hs-CRP in group CHD were higher than those in group C (P＜0.01).In group CHD, there was no obvious difference in the content of MBL between the patients with high level of hs-CRP(＞3 mg/L) and those with normal level of hs-CRP(＜3 mg/L) (P＞0.05).Conclusion MBL and hs-CRP may be involved in the occurrence and development of CHD via different mechanisms.%目的 研究冠心病(CHD)与甘露聚糖结合凝集素(MBL)基因多态性及其血浆MBL和高敏G-反应蛋白(hs-CRP)水平的关系.方法 收集100例CHD患者(CHD组)和60例健康人群(对照组)抗凝血,限制片段长度多态性法(PCR-RFLP)检测MBL ExonI 54位密码子基因点突变,ELISA法和免疫比浊法分别检测血浆MBL含量和高敏G-反应蛋白(hs-CRP)水平.结果 CHD组与对照组MBL基因ExonI 54位密码子点突变频率比较差异无统计学意义(P＞0.05).CHD组血浆MBI和hs-CRP含量均高于对照组(P＜0.01).而CHI组内血浆hs-CRP水平升高者(＞3 mg/L)与hs-CRP水平正常者(＜3 mg/L)MBL含量比较差异无统计学意义(P＞0.05).结论 MBL和hs-CRP可能以不同机制参与CHD发生、发展过程.