高效液相法测定胃康胶囊中芍药苷的含量

摘要

目的 建立胃康胶囊中芍药苷的含量测定方法.方法 以UltimateTM XB-C18(4.6mm×250 mm,5μm)为分析柱,异丙醇-甲醇-醋酸-水(2:25:2:71)为流动相;流速:1.0 ml·min-1;检测波长:230 nm;进样量10μl.结果 芍药苷对照品在10.80～97.20μg·ml-1范围内,浓度与峰面积呈良好的线性关系Y=4.74×103X+2.44×103,r=0.9999,平均回收率为99.2%,RSD=0.5%(n=9).结论 本法简便,准确,专属性强,可以作为该制剂的质量控制方法.%Objective To establish a method for determination Content of paeoniflorin in weikang capsule by HPLC.Methods The HPLC was conducted on a UltimateTM XB-C18 with isopropanol - methanol- acetate-water( 2:25:2:71 ) as mobile phase, flow rate 1.0 ml ·min-1, detected at 230nm;injection volume 10 μl.Results The calibration curve of paeoniflorin was linear with 10.80 ～ 97.20 μg·mL-1,linear relationship Y=4.74 × 103X+2.44 × 103,(r=0.9999),The average recovery of paeoniflorin was 99.2%, RSD = 0.5% (n=9). Conclusion The method is simple, accurate and suitable for the determination of weikang capsule.