Diagnostic value of Joint Detection of GP73 and AFP-L3 in Primar y Hepatic Carcinoma with Low Concentration of AFP

摘要

Objective:To explore the applicative value of serum Golgi protein (GP73) and alpha-protein-variant (AFP-L3) in the diagnosis of patients with primary hepatic carcinoma (PHC). n Methods:Totally 110 patients were enrolled, including 60 PHC patients with low concentration of AFP ((1.1 ~ 108.0μg/L)) and 50 patients with non-PHC digestive system diseases (20 cases of patients with chronic hepatitis, 15 patients with liver cirrhosis, 4 patients with bile duct cancer, 4 patients with gastric cancer, 4 patients with rectal cancer and 3 patients with colon cancer). In addition, 42 healthy people were selected as control group. GP73 was detected by enzyme-linked immunosorbent assay (ELISA) trace centrifugal column method was adopted for separation of AFP-L3. Luo’s chemiluminescence method was used to determine the total content of AFP and AFP-L3 in eluent for calculating the ratio of AFP-L3/AFP. n Results:The levels of serum GP73 and AFP-L3(%) in PHC group were signiifcantly higher than the other 2 groups (P<0.01) and the level of serum GP73 and AFP-L3 (%) in Non-PHC group were higher than those in healthy group (P < 0.01). ROC analysis showed that the area under ROC curve of single GP73 and AFP-L3(%) in diagnosis of PHC and non-PHC were 0.887 and 0.860, respectively. Additionally, the ROC analysis also showed that critical value of GP73 and AFP-L3 for the diagnosis of HPC were 83.78μg/L and 13.87%, respectively. The sensitivity of joint detection of serum GP73 or AFP-L3 was higher than detection of them alone (90.0 vs. 71.7 and 60.0, P<0.05) but the speciifcity was similar between single detection and joint detection (P>0.05). The positive predictive value and negative predictive value of joint detection of them was higher than single detections of them and there was signiifcant differences in negative predictive value (P<0.05) but no difference in positive predictive value (P>0.05). The overall response rate of joint detection of GP73 and AFP-L3 was higher than single detection of them (P<0.05). Conclusion:single detection of GP73 and AFP-L3 (%) were similar in the sensitivity and speciifcity in diagnosis of PHC patients with low concentration of AFP, which can be auxiliary diagnosis for PHC patients and joint detection of them is more reliable to diagnose PHC.