Objective: To compare a highly sensitive detection system with a traditional real-time PCR assay with external standards for HBV DNA quantiifcation.Methods: hTe concentrations of serum HBV DNA in 80 HBsAg-positive patients were determined by both a highly sensitive detection system and a traditional real-time PCR assay with external standards. Results: hTe positive rate determined by the highly sensitive system (92.5%) was signiifcantly higher than that by the traditional assay (50.0%) (P<0.01). The median HBV DNA concentration quantified by the highly sensitive system was 2.95×105 IU/mL, which was signiifcantly higher than that by the traditional assay (5.63×104 IU/mL) (P<0.01). hTere was a high correlation between HBV DNA concentrations (≥104 IU/mL) determined by the two different assays (R2=0.89,P<0.01). hTe positive rate of 26 HBeAg-negative patients determined by highly sensitive system (100.0%) was signiifcantly higher than that by traditional assay (11.5%) (P<0.01).Conclusion: hTe highly sensitive system is more suitable for HBV DNA quantiifcation of clinical serum samples with low virus load (<104 IU/mL).%目的：比较超敏HBV DNA检测系统与传统荧光定量PCR外标法定量检测血清乙肝病毒DNA，探讨其临床应用价值。方法：采用超敏PCR检测系统和传统荧光定量PCR外标法同时检测80例HBsAg阳性患者的血清HBV DNA浓度。结果：超敏法阳性检出率(92.5%)高于传统外标法(50.0%)(P<0.01)。超敏法阳性定量结果中位值为2.95×105 IU/mL，高于传统外标法(5.63×104 IU/mL)(P<0.01)，两种方法对高载量病毒(≥104 IU/mL)血清标本检测结果的相关性有统计学意义(R2=0.89，P<0.01)。超敏法对26例HBeAg阴性慢性乙肝患者的阳性检出率(100.0%)高于传统外标法(11.5%)(P<0.01)。结论：超敏HBV DNA定量检测系统检测灵敏度高，更适用于临床低载量病毒(<104 IU/mL)血清标本的检测。
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