多重实时PCR用于高危型人乳头瘤病毒感染检测的价值

摘要

Objective To explore the clinical significance of multiplex real-time PCR in detection of high-risk human paillomavirus(HPV) infection.Methods 178 suspected cases of HPV infection and cervical lesions were collected,and multiplex real-time PCR and HC2 method were respectively employed to dtect the high-risk HPV and their subtypes.Results Compared with multiplex real-time PCR and HC2 methods used in high-risk HPV detection,there was no statistical difference(P＞0.05),indicating that the two methods had similar detection performance.According to the two methods,positive rates of patients with LSIL,HSIL and squamous cell carcinoma were higher than those of cervicitis and condyloma,with statistical significant difference(P＜0.05).Conclusion Multiplex real-time PCR is suitable for large-scale screening and monitoring high-risk HPV persistent infection.%目的 探讨多重实时PCR技术的应用对高危型人乳头瘤病毒感染检测的临床意义.方法 选择疑似HPV感染和宫颈病变的病例178例,分别应用多重实时PCR检测法与HC2检测法检测高危型HPV及其亚型的感染情况.结果 多重实时PCR检测法与HC2检测法用于高危型HPV的检测,差异无统计学意义(P＞0.05),表明两种检测方法在检测效能上相近；两种检测方法中,低度鳞状上皮内病变(LSIL)、高度鳞状上皮内病变(HSIL)与鳞状细胞癌的阳性率均高于宫颈炎与湿疣,差异有统计学意义(P＜0.05).结论 多重实时PCR法适用于大规模筛查和高危型HPV持续感染的监测.