MALDI-TOF 质谱仪在临床常见细菌鉴定中的应用

摘要

目的：探讨两种质谱仪 Biotyper MS 和 Vitek-MS 系统在临床常规分离细菌鉴定中的应用价值。方法收集沈阳军区总医院2012年3月至2013年1月分离自血液、尿液、脑脊液、分泌物和痰液等标本的临床常见菌细菌149株（包括14个菌属和30个菌种）。同时采用两种 MALDI-TOF-MS 系统对上述菌株进行鉴定，结果与 Vitek2 compact 常规生化鉴定进行比较，对3种方法检测结果不一致菌株用16S rRNA 测序进行确认。结果在149株常见细菌中，Bruker Biotype 属和种的正确鉴定率分别为98％和96％，Vitek-MS 属和种的正确鉴定率分别为97％和95％。两者均无错误鉴定菌株。结论对该组常见细菌，两种质谱仪鉴定结果比较差异无统计学意义（P ＞0．05），均呈现出优异的鉴定水平，适合用于临床微生物的常规鉴定。%Objective To evaluated the application value of two kinds of mass spectrometer(MS)and Vitek MS system in the i-dentification of routinely isolated bacteria in clinic.Methods 149 strains of common bacteria(including 14 genera and 30 species)i-solated from blood,urine,cerebral spinal fluid,secretion and sputum samples in our hospital from March 2012 to January 2013 were collected and simultaneously identified by 2 kinds of matrix-assisted laser desorption ionization-time of flight mass spectrometer (MALD-TOF-MS).The identification results were compared with those identified by the conventional biochemical identification (Vitek2 compact).The strains with the inconsistent results identified by 3 kinds of method were confirmed by 16S rDNA gene se-quencing.Results Among 149 common bacteria,the correct identification rates of genus and species by the Bruker Biotyper MS were 98% and 96% respectively and which by the Vitek MS system were 97% and 95% respectively.There were no misidentified bacterial strains by these two kinds of MS.Conclusion No statistical difference in the identification results was observed between these two kinds of MS system(P >0.05).Both exhibit excellent identification level and are suitable for the routine laboratory iden-tification of clinical microorganism.