“自上而下”方法在评定生化检测指标测量不确定度中的应用

摘要

目的：探讨“自上而下”方法在评定临床生化指标的测量不确定度中的应用。方法利用室内质控和室间质评数据，采用“自上而下”方法评定26个生化指标的精密度和正确度，综合以上2个变量合成测量不确定度。结果18个生化指标的不确定度符合目标不确定度要求，约占所有评估项目69．2％，TBIL、清蛋白、CK、钙、镁5个指标不符合目标不确定度要求，约占总评估项目19．2％。结论采用“自上而下”方法评定生化指标的测量不确定度有效、可行。%Objective To investigate the application of “top-down” method in assessment of measurement uncertainty of bio-chemical detection indicators .Methods The assessment data of internal quality control and external quality and “top-down”method were used to assess the precision and accuracy of 26 biochemical indicators ,and the two variables above were combined to calculate the measurement uncertainty .Results Uncertainty of 18 biochemical indicators was compatible with the target uncertainty ,ac-counting for approximately 69 .2% of all assessment projects .Five indicators ,such as TBIL ,albumin ,CK ,calcium and magnesium , was not compatible with the target uncertainty ,accounting for approximately 19 .2% of all assessment projects .Conclusion The“top-down” method is effective and feasible for assessment of measurement uncertainty of biochemical detection indicators .