大黄黄连泻心汤、理中丸对消炎痛型及水浸应激型胃溃疡寒热证模型大鼠胃组织EGF、TGF-α含量的影响

摘要

Objective: To explore the forming mechanism of gastric ulcer model induced by indomethacin or water immersion stress combined with cold or heat syndrome. And to observe the basic and objective regularity of " formula - syndrome corresponding" of cold or heat formula on gastric ulcer model combined with cold or heat syndrome. Methods: Adopting cold or heat factors combined with injecting indomethacin into abdominal cavity or water immersion stress methods to establish gastric ulcer model combined with cold and heat syndrome. U-sing ELISA to detect the influence of Dahuang Huanglian Xiexin Decoction and Lizhong Pill on EGF and TGF - α contents in gastro - tissue. Results: Compared with the blank group, the contents of EGF and TGF - α in stomach - tissue of every modle - group decreased remarkably. (P < 0. 05 or P < 0. 01) Dahuang Huanglian Xiexin Decoction can increase the contents of EGF and TGF - α in stomach - tissue of heat - ulcer group obviously . Lizhong Pill can increase the contents of EGF and TGF - α in stomach - tissue of cold - ulcer group strikingly. (P <0. 05 or P <0. 01) Conclusions:On the basis of the theory of "formula - syndrome corresponding" , verifying the success of duplicating "disease - syndrome combination model" , that is gastric ulcer model combined with cold and heat syndrome. The therapeutic action of Dahuang Huanglian Xiexin Decoction on heat - gastric ulcer and prevention of heat stress gastric ulcer formation flooding or Lizhong Pill on cold - gastric ulcer and prevention of cold stress gastric ulcer formation flooding both can be achieved by increasing the contents of EGF and TGF - α in stomach - tissue.%目的:探讨消炎痛型、水浸应激型胃溃疡中医寒热证模型的形成机制及寒热方剂对胃溃疡寒热证模型的基本作用机理和方证相应的客观规律.方法:采用寒热因素结合腹腔注射消炎痛和水浸应激法,分别建立大鼠胃溃疡寒、热证模型,用酶联免疫法检测大黄黄连泻心汤、理中丸对受试大鼠胃组织中EGF和TGF-α含量的影响.结果:各模型组大鼠胃组织EGF和TGF-α含量显著降低,与空白组比较具有显著性差异(P＜0.05或P＜O.01);大黄黄连泻心汤可显著升高热性溃疡组大鼠胃组织EGF和TGF-α的含量,理中丸可显著升高寒性溃疡组大鼠胃组织EGF和TGF-α的含量(P＜0.05或P＜0.01).结论:通过"方证相应"理论验证了"病证结合"的胃溃疡寒热模型复制成功,大黄黄连泻心汤可以治疗热性消炎痛型胃溃疡,预防热性水浸应激型胃溃疡的形成;理中丸可以治疗寒性消炎痛型胃溃疡,预防寒性水浸应激型胃溃疡的形成,均可通过升高大鼠胃组织中EGF和TGF-α的含量而实现.