二氢槲皮素对 H2 O2诱导 H9 c2心肌细胞损伤的保护作用

摘要

Objective:To investigate the protective effects of dihydroquercetin against H 2 O2 -induced injury in H9c2 cardiomyoblasts.Methods:A model of H2O2 -induced injury in H9c2 cardiomyoblasts was established. Cell viability was determined colorimetrically by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazo-lium (MTT) assay.The supernates and cells were collected , respectively, after the different treatments for measuring the LDH and MDA levels, as well as SOD,CAT, and GSH-Px activity with the corresponding de-tection kit according to the manufacturer ’ s instructions .Results:H9 c2 cells were pretreated with dihydroquer-cetin for 12 h before exposure to 150 μM for 6 h.H2 O2 treatment caused severe injury to H9c2 cardiomyo-blasts.Dihydroquercetin pretreatment could attenuate H 2 O2 -induced injury , decrease the levels of LDH leakage and MDA, and increase the activities of SOD, CAT, and GSH-Px with a dose-dependent.Conclu-sion:Dihydroquercetin showed significantly protective effects against H 2 O2 -induced injury in H9c2 cardio-myoblasts in dose-dependent .The mechanism of this may be due to its increasing the activities of endogenous antioxidant enzymes , as well as improving the stabilization of myocardial cells .%目的：研究二氢槲皮素对H2 O2诱导H9c2心肌细胞损伤的保护作用。方法：建立H2 O2诱导H9c2心肌细胞损伤模型；利用四甲基偶氮唑蓝（MTT）法考察预孵育二氢槲皮素对H2O2诱导H9c2心肌细胞损伤细胞存活率的影响；利用试剂盒检测培养液中乳酸脱氢酶（ LDH），细胞内丙二醛（ MDA），过氧化氢酶（ CAT ）、超氧化物歧化酶（ SOD ）及谷胱甘肽过氧化物酶（ GSH-Px ）含量的变化。结果：不同浓度的二氢槲皮素预处理4、12、24 h均能够提高细胞的存活率，且呈剂量依赖性，其中预处理12 h的保护作用最为明显。与正常对照组相比，模型组的存活率下降，LDH的漏出量增大，细胞内MDA的含量增大，CAT、SOD、GSH-Px的活力明显降低。用二氢槲皮素预处理12 h能显著地减少LDH的漏出量，降低细胞内MDA的含量，提升细胞内CAT、SOD、GSH-Px的活力，并呈剂量依赖性。结论：二氢槲皮素对H2 O2诱导H9 c2心肌细胞损伤具有显著保护作用，且呈剂量依赖性，其作用机制可能与其能够提高细胞内抗氧化酶活性，增强细胞稳定性有关。