HAART对HIV辅助受体的影响研究

摘要

目的 探讨在HIV-1感染过程中,高效抗反转录病毒治疗(highly active antiretroviral therapy,HAART)与病毒嗜性的关系.方法 从治疗前和治疗24周患者的外周血单个核细胞中提取基因组DNA,克隆HIV-1 env区的C2-V5区并测序.基于V3区氨基酸序列,通过Gen02pheno和PSSM软件预测共受体嗜性.结果 经HAART后,携带CXCR4和CCR5双受体的病毒载量都有所下降,HAART 24周R5病毒的表达率高于X4,且阳性电荷和净电荷都明显降低(P＜0.05).结论 HAART能够有效地抑制X4和R5毒株,并优先抑制X4毒株,有助于改善疾病预后,可依此作为CCR5抑制剂治疗失败的患者制定新方案的参考.%Objective To investigate the relationship between highly active antiretroviral therapy (HAART) and viral tropism during HIV-1 infection. Methods Genomic DNA was extracted from peripheral blood mononuclear cells (PBMCs) of patients before HAART and at week 24 of HAART. C2-V5 regions of HIV-1 env were cloned and sequenced. Co-receptor tropism was predicted by Geno2pheno and PSSM software based on amino acid sequence of V3 region. Results After HAART, viral loads were declined in viral strains carrying CXCR4 and CCR5 receptors. At week 24 of HAART, expression rate of R5 virus was higher than that of X4 virus, and positive charge and net charge significantly reduced (P<0.05). Conclusions HAART can effectively suppress X4 and R5 strains, with priority to suppressing X4 strains. This helps to improve the prognosis of the disease, and provide an evidence for the patients failing treatment with CCR5 inhibitor to develop new regimens.