河南省有偿供血人群HIV/HCV混合感染者HIV-1细胞嗜性的研究

摘要

Objective To investigate HIV-1 cell tropism of the population coinfected with HIV and HCV through paid blood donation in Henan province. Methods Totally 161 HIV-1 sero-positive blood samples were collected from individuals who were coinfected with HIV and HCV through paid blood donation in Henan province. HCV antibody was determined using enzyme-linked immunosorbent assay, and HIV/HCV coinfection was confirmed by the detection of HCV RNA. HIV-1 env C2V3 gene was amplified using nested-PCR and the subtype characterization was analyzed after sequencing. Co-receptor usage was predicted using biosoftware WebPSSM, and distribution of tetramer compositions on the tip of V3 loop was analyzed. Results Among the total 161 individuals infected with HIV-1 through paid blood donation, HCV sero-prevalence was 87.58% (141/161), and the positive rate of HCV RNA was 56.52% (91/161). The most predominant HIV-1 subtypes in both HIV monoinfection group and HIV/HCV coinfection group were B (Br) (96% and 98.5%, respectively), and the main tip motifs of V3 loop were GPGR (33.4% and 33.8%), GPGQ (22.0% and 19.1%) and GPGK (14.0% and 19.1%). Co-recepter CCR5 was mainly used in both HIV monoinfection group and HIV/HCV coinfection group, and the HIV-1 cell tropism between the two groups was not significantly different (P=0.971). Conclusions The cell tropism of HIV-1 has no correlation with HCV infection. The decetion of HCV RNA helps to clarify the current infection situation in HIV/HCV coinfected population.%目的 探讨河南省有偿供血人群HIV/HCV混合感染者体内HIV-1细胞嗜性的分布情况.方法 选取河南省经有偿供血途径感染HIV-1的161例感染者,采用酶联免疫法检测HCV抗体,并通过HCV核酸检测明确HIV/HCV混合感染.nested-PCR扩增env C2V3区,测序并分析HIV -1流行亚型,使用在线预测工具WebPSSM预测辅助受体的使用情况,分析V3环顶端四肽.结果 河南省经有偿供血途径感染HIV-1人群的抗HCV阳性率为87.58％( 141/161),HCV RNA阳性率为56.52％(91/161),HIV单独感染组和HIV/HCV混合感染组HIV-1均以B(B')亚型多见(96％和98.5％),V3环顶端四肽主要为GPGR(33.4％和33.8％)、GPGQ(22.0％和19.1％)和GPGK( 14.0％和19.1％).2组感染者的HIV-1均以使用CCR5辅助受体为主,HIV-1细胞嗜性在2组中的分布差异无统计学意义(P=0.971).结论 HCV混合感染不影响HIV-1的细胞嗜性.HCV RNA检测有助于明确HIV/HCV混合感染者现症感染情况.