苦参碱与人血清白蛋白和牛血清白蛋白相互作用的光谱学研究

摘要

The interaction between matrine (MAT) and human serum albumin (HSA) and bovine serum albumin (BSA) were investigated by fluorescence spectroscopy,UV absorption spectroscopy and circular dichroism spectroscopy with the simulated physiological condition. The experiment results of show UV absorption spectroscopy and fluorescence spectroscopy that matrine can react with HSA or BSA and form a kind of new compound at the ground state and prove that the flurescence quenching of HSA or BSA by matrine is dynamic quenching,and at different temperature,quenching constants are respectively HSA:4.60×103,1.71×104,3.07×104L/mol and BSA:3.17×104﹑3.91×104and 4.48×104L/mol; binding constants are respectively HSA:5.999×105﹑3.097×106,6.667×107L/mol and 1.007×105,1.163×105,1.582×105L/mol, the number of binding sites is approximately equal to 1. The calculation results of thermodynamic parameters display that the reaction is hydrophobic effect and is sponta-neous reaction between matrine and HSA or BSA.%在模拟生理pH条件下,采用荧光光谱法、紫外-可见吸收光谱法和圆二色谱法研究了苦参碱与人血清白蛋白(Human serum albumin,简称HSA)和牛血清白蛋白(Bovine serum albumin,简称BSA)的相互作用.紫外吸收光谱法和荧光光谱法的试验结果表明,苦参碱对HSA和BSA相互作用在基态时形成复合物,因此对HSA和BSA均具有一定的荧光猝灭作用,其猝灭方式为静态猝灭,在22、27和37℃时其猝灭常数分别为HSA 3.07×104、1.71×104、4.60×103L/mol和BSA 4.48×104、3.91×104和3.17×104L/mol;结合常数分别为HSA 5.999×105、3.097×106、6.667×107L/mol和1.007×105、1.163×105、1.582×105L/mol;结合位点数基本维持不变.而圆二色谱法和热力学参数的计算结果表明苦参碱与HSA及苦参碱与BSA两者之间的作用力为疏水作用且为自发反应.