目的：建立高效液相色谱-二极管阵列检测器( HPLC-DAD)法同时测定常见染红中药中非法添加的20种人工合成色素。方法采用70%甲醇超声提取样品,通过Agilent TC-C18反相色谱柱(4.6 mm×250 mm,5μm)分离,以甲醇-0.02 mol·L-1醋酸铵溶液为流动相进行梯度洗脱,于210~650 nm波长范围用DAD检测,用保留时间结合待测物的紫外吸收光谱验证。结果同时分离并检测出20种常用红色及橙色系色素,市售西红花、朱砂中检出酸性橙域及808猩红。结论该方法可同时检测常见染红中药中常用的非法添加人工合成色素,样品处理简便,方法快速,结果准确可靠,重复性好。%Objective To develop a HPLC-DAD method for the simultaneous determination of 20 synthetic pigments in dyed Traditional Chinese Medicine. Methods The samples were extracted with ultrasonic by 70% methanol, separated on a Agilent TC-C18 column ( 4. 6 mmí250 mm, 5 μm ) by gradient elution using a mobile phase made up of methanol and 0. 02 mol·L-1 ammonium acetate, and detected at 210-650 nm. The retention times and ultraviolet absorption spectrums were used to text and verify. Results The twenty red and orange synthetic pigments were separated and detected. OrangeIIsodium salt and 808 scarlet were detected in some batch of Croci stigma and Cinnabaris. Conclusion The method is simple, rapid, accurate, repeatable for determining twenty synthetic pigments in dyed Traditional Chinese Medicine.
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