狭叶崖爬藤对类风湿关节炎大鼠的免疫调节作用∗

摘要

目的：探讨狭叶崖爬藤在治疗类风湿关节炎中的免疫调节作用。方法60只SD大鼠按随机数字法分为正常对照组，模型对照组，狭叶崖爬藤大、中、小剂量组(200，100，50 mg•kg－1)，雷公藤多苷组(给予雷公藤多苷)，每组10只。除正常对照组外，其他SD大鼠用牛Ⅱ型胶原蛋白( CⅡ)诱导建立胶原性关节炎( CIA)模型，观察对CIA模型大鼠脏器指数、血清中白细胞介素( IL)-1β、IL-6、肿瘤坏死因子-α( TNF-α)和抗CⅡ含量的影响。结果狭叶崖爬藤小剂量组、中剂量组肾脏指数与模型对照组比较均差异有统计学意义(P<0.05)。狭叶崖爬藤大剂量组脾脏指数与正常对照组比较差异有统计学意义( P<0.05)。模型对照组IL-1β、IL-6、TNF-α和抗CⅡ含量均显著高于正常对照组，而狭叶崖爬藤中剂量组、大剂量组与模型对照组比较，血清中IL-1β、IL-6、TNF-α水平均降低，差异有统计学意义( P<0.01)。狭叶崖爬藤小剂量组、中剂量组、大剂量组与模型对照组比较能显著降低抗CⅡ抗体含量。结论狭叶崖爬藤有免疫调节作用，可用于治疗类风湿关节炎，具有开发利用价值。%Objective To investigate the immunomodulatory effects of Tetrastigma hypoglaucum Planch in the treatment of rheumatoid arthritis. Methods Sixty SD rats were ramdomly divided into normal control group, model control group,low-,middle-and high-dose Tetrastigma hypoglaucum Planch groups (50,100 and 200 mg.kg-1,respectively),and tripterygium glycosides tablet group. Except for normal control group,rheumatoid arthritis model was established by using bovine typeⅡcollagen in SD rats of the other groups. Organ index,plasma levels of IL-1β,IL-6,TNF-αand anti-CⅡin rat rheumatoid arthritis models were evaluated. Results The kidney index of the low-dose/middle-dose Tetrastigma hypoglaucum Planch groups were significantly different from that of the model control group (P<0.05). Significant difference was also found in the spleen index between the high-dose Tetrastigma hypoglaucum Planch group and the normal control group ( P<0.05) . The plasma levels of IL-1β,IL-6,TNF-αand anti-CⅡantibody were significantly higher in the model control group than in the normal control group. Meanwhile, the plasma IL-1β, IL-6 and TNF-α levels of the middle-dose/high-dose Tetrastigma hypoglaucum Planch groups were significantly lower than those of the model group ( P<0.01) . The levels of anti-CII antibody were significantly lower in the low-,middle-and high-dose Tetrastigma hypoglaucum Planch groups than in the model control group. Conclusion It is worthwhile to explore and develop Tetrastigma hypoglaucum Planch since it possesses immunomodulatory effects and may be applied in the treatment of rheumatoid arthritis.