银杏叶总内酯对照提取物在银杏叶含量测定中的应用

摘要

Objective To explore the feasibility of using the quantitative reference extract of ginkgo leaf total lactones instead of single component reference for the quantitative assay of Ginkgo Folium.Methods HPLC-ELSD method was performed by using a Diamonsil C18 column (250 mm×4.6 mm,5 μm) with methanol-water as the mobile phase at the gradient elution mode.Flow rate was 1.0 mL·min-1.The parameters of ELSD detector were as follows,the drifit tube temperature was 105 ℃,and the flow rate of nitrogen(N2) was 3 L·min-1.Results The linear ranges of ginkgolide A,ginkgolide B,ginkgolide C,and bilobalide were 0.735-5.879 μg (r=0.999 6),0.404-6.060 μg (r=0.999 6),0.296-4.439 μg (r=0.999 6),and 1.001-6.006 μg (r=0.999 7),respectively.The recoveries and RSD of the four components were 95.6% (4.0%),97.3% (4.5%),99.3% (5.0%),and 100.4% (2.1%),respectively.Conclusion The quantitative reference extract of ginkgo leaf total lactones can be used as the substitute for the determination of terpene lactones.%目的 探讨选择银杏叶总内酯对照提取物替代单体成分对照品测定银杏叶中萜内酯类成分含量的可行性.方法 以定值的银杏叶总内酯对照提取物为对照,采用高效液相-蒸发光散射检测(HPLC-ELSD)法,色谱柱为Diamonsil C18(250 mm×4.6 mm,5 μm),流动相为甲醇-水,梯度洗脱,流速为1.0 mL·min-1,ELSD检测器:漂移管温度105 ℃;氮气(N2):3.0 L·min-1.结果 银杏内酯A、银杏内酯B、银杏内酯C、白果内酯的进样量与峰面积分别在0.735~5.879 μg(r=0.999 6)、0.404~6.060 μg(r=0.999 6)、0.296~4.439 μg(r=0.999 6)、1.001~6.006 μg(r=0.999 7)范围内呈良好的对数线性关系;平均回收率分别为95.5%,97.4%,99.3%,100.4%,RSD依次分别为4.0%,4.5%,5.0%,2.1%.结论 银杏叶总内酯对照提取物可以替代相应的单体对照品用于银杏叶中萜内酯类成分的定量测定.