珊瑚姜油对宫颈癌HeLa细胞增殖及凋亡的影响

摘要

Objective To investigate the effect of Zingiber corallinum oil ( ZCO ) on apoptosis and proliferation of cervical carcinoma cell line HeLa. Methods HeLa cells were treated with different concentrations of ZCO(5-80 mg·L-1)in vitro. Cytotoxicity rate was determined by CCK-8 assay. The morphological changes was observed using inverted microscope after AO/EB staining. Caspase-3 activities were measured with a colorimetric method. Protein level of hsp-70 were detected by Western blotting. Cell cycle and apoptosis were analyzed by flow cytometer ( FCM ) . Results ZCO exhibited effect of proliferation inhibition and apoptosis-inducing on the growth of HeLa cells in a dose-dependent manner. Caspase-3 activities increased in a dose-dependent manner while the expression of hsp-70 decreased. Cell cycle was arrested in G2/M phase. Conclusion ZCO exhibites a marked effect of proliferation inhibition and apoptosis-inducing on HeLa cells. The mechanism of ZCO might be activating the key enzyme in apoptotic pathway, so that the expression of hsp-70 is down-regulated, and cell cycle is arrested in G2/M phase.%目的 探讨珊瑚姜油(ZCO)对宫颈癌HeLa细胞增殖及凋亡的影响及其作用机制.方法 5~80 mg·L-1 ZCO体外作用于HeLa细胞.CCK-8检测细胞毒性;吖啶橙/溴化乙啶(AO/EB)染色,倒置显微镜观察细胞形态变化;比色法检测caspase-3酶活性;Western blotting检测hsp-70表达;流式细胞仪检测细胞凋亡及周期.结果 ZCO以时间-剂量依赖性方式抑制HeLa细胞株增殖,镜下见典型细胞凋亡形态,caspase-3酶活性亦呈剂量依赖性增加.随药物浓度增加,hsp-70蛋白表达量逐渐减少,G2期细胞逐渐增加,细胞阻滞在G2/M期.结论 ZCO对HeLa细胞株具有抑制增殖及促凋亡作用,其机制是激活凋亡关键酶,使hsp-70蛋白表达量逐渐下降,使细胞周期阻滞在G2/M期.