苍柏纳米乳在体肠吸收机理的研究

摘要

选用大鼠在体小肠单向灌流法为模型，以苍柏纳米乳灌流液中盐酸小檗碱的含量为指标，考察苍柏纳米乳在大鼠小肠不同肠段的吸收情况；并探讨P-糖蛋白（P-gp）抑制剂盐酸维拉帕米对苍柏纳米乳的肠吸收情况的影响。苍柏纳米乳中盐酸小檗碱在大鼠十二指肠段的吸收参数Ka、Papp值显著高于其在空肠、回肠、结肠段的吸收。苍柏纳米乳中盐酸小檗碱在吸收过程中存在高浓度饱和现象，且P-gp抑制剂盐酸维拉帕米对苍柏纳米乳小肠吸收情况的影响没有显著性差异。苍柏纳米乳的最佳吸收肠段为十二指肠，空肠、回肠次之，结肠吸收最差。苍柏纳米乳中盐酸小檗碱的吸收过程为主动转运，且不受P-gp抑制剂的影响。%The single-pass intestinal perfusion method of rats was chosen as the model,the content of Berberine Hydrochloride in the pale cypress of Cangbai nanoemulsion was taken as the index,and the intestine absorption situation was studied. The influence of P-gp protein inhibitor,verapamil hydrochloride,on the pale of the intestinal absorption of Cangbai nanoemulsion was also investigated in this study. The absorption parameters Ka,Papp values of berberine hydrochloride in duodenal of rats were significantly higher than that of jejunum,ileum,colon. High saturation phenomenon existed in the absorption process of berberine hydrochloride,and the influence of P-gp protein inhibitor, Verapamil Hydrochloride,on the intestinal absorption was not obvious. The best absorb intestinal segment of Cangbai nanoemulsion was duodenum,jejunum and ileum were much worse and colon absorption was the worst. The absorption process of berberine hydrochloride was an active transport,and it was not affected by P-gp protein inhibitors.