145例胃腺癌P21和P53免疫组化分析

摘要

Objective: To study the expression of cancer gene product and anti-cancer gene product in the same gastric carcinoma.Methods:Immunohistochemical staining was used to determine the expression of P21 and P53 in 10 normal stomach mucous membrane.10 atrophic gastritis. 10 intestinal metaplasia and 145 gastric carcinoma. Results: No P21 and P53 were expressed in the 10 normal stomach mucous membrane. 10 atrophic gastritis. 10 intestinal metaplasia samples. The positive incidence of P21 and P53 expression in the gastric carcinoma were 55.2% and 51 .2% respectively. The dexpression of P21 and P53 correlated to the degree of gastric carcinoma differtiation .P53 was located in cell nuclei and P21 located on the cell membrane or cell starch in gastric carcinoma.Conclusion:These results suggest that P21 and P53 may be used to determine the prognosis of gastric carcinoma patients.%目的：研究同一胃癌中癌基因和抑癌基因蛋白产物的表达。方法：应用免疫组织化学SP方法检测10例正常胃粘膜、10例萎缩性胃炎、10例肠上皮化生的胃壁组织和145例胃癌组织中P21和P53蛋白的表达水平。结果：10例正常胃粘膜、10例萎缩性胃炎、10例肠上皮化生的胃壁组织中未发现P21和P53。145例胃癌组织中P21和P53的阳性检出率分别为55．2%和51.2％。表达阳性的P53定位于肿瘤细胞核内，P21定位于细胞膜或细胞浆上。P21和P53的阳性表达率与胃癌的分化程度呈正相关。结论：检测P21和P53有助于评估胃癌的预后。