非对称性二甲基精氨酸在慢性心力衰竭发病过程中的作用机制研究

摘要

Objective:To investigate the relevance between asymmetric dimethylarginine and nitric oxide during the pathogenesis of chronic heart failure patients,furtherly to study the molecular mechanism of both in chronic heart failure progression.Method:To test serum asymmetric dimethylarginine and NO concentrations of 60 patients with CHF and 60 healthy in control group,and test left ventricular parameters through ultrasound of CHF group and control group of healthy,and analyse the correlation.Then test the expression levels of DDAH gene and endothelial nitric oxide synthase gene in two groups by the fluorescence quantitative of PCR.Result:The asymmetric dimethylarginine and NO level of patients with chronic heart failure of cardiac function grading was significanctly different from the control group (P＜0.05).Asymmetric dimethylarginine levels were positively related to NYHA grading,and related negatively to left ventricular ejection fraction,in contrast to NO.In gene level matters,DDAH and nitric oxide synthase gene were negatively related to NYHA grading.Conclusion:Asymmetric dimethylarginine level is positively related to NYHA grading,which can be used as the index in the diagnosis of chronic heart failure.%目的:探讨慢性心力衰竭患者发病过程中非对称性二甲基精氨酸与一氧化氮的相关性,进一步研究两者在慢性心力衰竭发病进程中的分子机制.方法:测定60例CHF患者以及60例健康对照组血清中非对称性二甲基精氨酸浓度和NO的含量,同时通过超声心动图测定CHF患病组和健康对照组的左心室参数,分析其相关性.然后利用荧光定量PCR检测DDAH基因及一氧化氮合酶基因在两组中的表达水平.结果:不同心功能分级的慢性心力衰竭患者非对称性二甲基精氨酸水平及NO含量与对照组相比,具有统计学意义(P＜0.05).非对称性二甲基精氨酸水平及与不同心功能分级呈正相关,与左心室射血分数呈负相关,NO相反.从基因水平角度分析,DDAH及一氧化氮合酶基因随着心功能分级升高,基因表达水平呈下降趋势.结论:非对称性二甲基精氨酸水平与心功能级别呈正相关,能够作为慢性心力衰竭的诊断指标.