乙型肝炎病毒抗原抗体标志物与 HBV-DNA 联合检测的相关性分析

摘要

目的：分析乙肝五项及前S1抗原联合检测与HBV-DNA结果的一致性，探讨三者的相关性。方法：对508名门诊及住院病人的血清同时进行ELISA法检测乙肝五项、前S1抗原及荧光定量PCR法检测HBV-DNA，对检测结果进行对比分析。结果：乙肝五项不同模式间前S1抗原与乙肝DNA检测阳性率存在一定的差异，大三阳的前S1抗原和DNA检测阳性率分别为86．2％、100％，小三阳的检测阳性率分别为84．0％、92．0％，但具有一致性。结论：HBV前Sl抗原和HBV-DNA在各组中阳性检出率有较高的一致性，前Sl抗原在一定程度上可替代HBV-DNA。前Sl抗原与乙肝血清标志物联合检测能为乙肝患者病毒复制、肝功能损伤提供有价值的实验室依据，同时有助于慢性乙肝患者疗效考核和预后判断。%Objective:To study the concordance of detection among HBV markers ,Pre S1 antigen and HBV-DNA, probe into the relativity between them .Method: Used Elisa to examine HBsAg , HBsAb, HBeAg HBeAb, HBcAb and preS1Ag of 508 patient's sera, and PCR to examine HBV-DNA, then com-pared and analyzed the results .Result:Difference of positive rate existed between Pre S 1 antigen and HBV DNA among various mode of HBV markers .In the HBeAg positive row , preS1Ag positive ratio was 86.2%and that of HBV-DNA was 100%.Both preS1Ag and HBV-DNA reflected the activity and replication of HBV.In the HBeAg negative row , preS1Ag and HBV-DNA positive ratios were 84.0%and 92.0%.The positive ratio of HBV-DNA was consistent with preS1Ag in detection(P>0.05).Conclusion:PreS1Ag can reflect the existence of HBV replication sensitively and can substitute HBV DNA .The diagnosis will be exact-ly if use the methods of preS 1Ag and hepatitis B serum markers in combination .