Ion Torrent测序检测非小细胞肺癌外周血 DNA 的临床价值

摘要

Objective:To investigate the clinical value of ion torrent sequencing with DNA for non -small cell peripheral lung cancer .Method:80 cases were divided into two groups , each with 40 cases, the observation group were used three generations with ion torrent sequencing technology ,the control group were used the first-generation sequencing technology method with Sanger sequencing , then sequencing proceeds before and after surgery and EGFR by ion torrent K-ras results and statistics sequencing EGFR-positive a two-year survival were compared .Result:The observation group with no surgery of EGFR-positive and K-ras mutation positive detection rate were significantly higher than those of control group (P<0.05), the ob-servation group with recurrence of EGFR-positive group and K-ras mutations were significantly higher than those of control group ( P<0.05) , 1-year survival rate in the observation group was significantly higher than that of control group (P<0.05).Conclusion:Ion torrent sequencing technology to measure peripheral blood DNA with non-small cell lung cancer patients can more accurately determine the genetic type of the patient , and thus provide the basis for molecular targeted therapy .%目的：探讨Ion Torrent测序检测非小细胞肺癌外周血DNA的临床价值。方法：选择患者80例，分为两组，各40例，观察组利用三代测序技术 Ion Torrent检测，对照组使用第1代测序技术Sanger末端终止法进行测序，比较手术前后通过Ion Torrent测序所得EGFR和K－ras结果，并统计两组测序EGFR阳性1年生存情况。结果：未手术者观察组EGFR阳性和K－ras突变阳性检出率均显著高于对照组（P＜0．05），术后复发患者观察组EGFR阳性和K－ras突变阳性检出率均显著高于对照组（P＜0．05），观察组1年生存率显著高于对照组（P＜0．05）。结论：对非小细胞肺癌患者使用Ion Torrent测序技术测定外周血DNA，能更准确的确定患者基因类型，从而为分子靶向治疗提供依据。