大麻二酚抑制小鼠脊髓损伤后炎症反应及促进功能恢复的研究

摘要

Objective:To investigate the inhibition of inflammation by cannabidiol in a mouse model of spinal cord injury and study its mechanism.Methords: 24 male C57Bl/6 mice were randomized into 3 groups: sham-operation group, control group and medication group.We established an spinal cord injury model by clip method, the mice in sham-operate group and control group were administered with a mixture of 1% of tween 80 and sterile saline (0.25mL/10g) and medication group were treated with cannabidiol (30mg/kg;was dissolved in a mixture of 1% of Tween 80 and sterile saline (1.2mg/mL)) by intraperitioneal injection at 1h、12h、24h、36h、48h、60h after operation.72h after operation , BMS test was used to evaluate the neurological score, the expression levels of HMGB1 and TNF-αin spinal cord tissue were assayed by ELISA.Rsults: The BMS test showed that medication group had a higher score than the control group(P<0.05).The expression levels of HMGB1 and TNF-α in medication group were significant reduced as comparison with control group P<0.05), but significant increased than the sham-operation group(P<0.05).Conclusion: Cannabidiol can inhibit inflammation in a mouse model of spinal cord injury via inhibiting the expression of HMGB1.%目的:探讨大麻二酚抑制小鼠急性脊髓损伤后炎症反应及其机制.方法:将C57/BL6小鼠随机分为3组(假手术组、对照组和用药组),每组8只,共24只.用药组和对照组小鼠用钳夹法夹断小鼠脊髓,假手术组不夹断脊髓,术后每日为小鼠挤压膀胱,帮助排尿.术后1h、12h、24h、36h、48h、60h给药,依据小鼠体重,对照组和假手术组腹腔注射安慰剂(0.25mL/10g,1%吐温80+生理盐水),用药组予以腹腔注射30mg/kg大麻二酚(1.2mg/mL,溶剂为1%吐温80+生理盐水).术后72h评价小鼠脊髓神经功能,行BMS(Basso mouse scale,BMS)评分,并通过ELISA检测组织中高迁移率族蛋白1(HMGB1)和肿瘤坏死因子-α(TNF-α)含量.结果:BMS(Basso mouse scale,BMS)评分,假手术组>用药组>对照组(P<0.05);脊髓组织中HMGB1和TNF-α含量均为假手术组<用药组<对照组(P<0.05).结论:大麻二酚能通过抑制脊髓损伤小鼠模型HMGB1表达来抑制小鼠炎症反应,从而对脊髓损伤小鼠脊髓神经功能保护.