Objective To investigate the correlation between methylation of transforming growth factorβreceptorⅠ,Ⅱ(TβRⅠ,Ⅱ) gene and pathogenesis, development of endometrial cancer (EC).Methods The modified methylation specific PCR ( MSP) was used to detect the methylation status of TβRⅠ,Ⅱgene promoter in 60 cases of EC tissues and 60 cases of adjacent non-cancerous tissues.The correlation between the methylation of two gene promotors and clinical indexes was analyzed.Results The mathylation rate of TβRⅠin EC tissues and adjacent non-cancerous tissues was 75%(45/60) and 45% ( 27/60 ) , respectively. The methylation rate of EC tissues was significantly higher than that of adjacent non-cancerous tissues (χ2 =11.25, P <0.01).The mathylation rate of TβRⅡ in EC tissues and adjacent non-cancerous tissues was 68.3%(41/60) and 36.7%(22/60),respectively.The methylation rate of EC tissues was significantly higher than that of adjacent non-cancerous tissues (χ2 =12.06, P <0.01 ) .However there was no correlation between the methylation of two gene promotors and clinical indexes ( P >0.05).Conclusion The methylation of TβRⅠand TβRⅡpromoters may be correlated to the pathogenesis of EC.%目的:探讨TβRⅠ和TβRⅡ基因甲基化与子宫内膜癌发生发展的关系。方法应用改进的甲基化特异性聚合酶链式反应( MSP)技术检测60例子宫内膜癌组织及60例癌旁正常组织中TβRⅠ、TβRⅡ基因启动子甲基化情况,分析两基因启动子甲基化与各临床指标之间的关系。结果 TβRⅠ在子宫内膜癌组和癌旁正常组的甲基化率分别为75 q.0%(45/60)和45.0%(27/60),子宫内膜癌组的甲基化率显著高于癌旁正常组(χ2=11.250, P =0.001)。 TβRⅡ在子宫内膜癌组和癌旁正常组的甲基化率分别为68.3%(41/60)和36.7%(22/60),子宫内膜癌组的甲基化率显著高于癌旁正常组(χ2=12.063, P =0.000)。两基因启动子甲基化与临床指标差异无统计学意义(P>0.05)。结论 TβRⅠ和TβRⅡ基因启动子甲基化可能与子宫内膜癌的发生有关。
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