为探索不同NaCl浓度胁迫对离体条件下杂交酸模97-1种子萌发及幼苗生理指标的影响,利用组织培养方法研究NaCl盐胁迫对杂种酸模97-1萌发生长的影响及其生理响应,结果表明:种子发芽率与NaCl浓度呈极显著负相关,种子萌发的耐盐临界值约为0.93%,NaCl浓度在1.6%～1.8%时发芽率＜10%;随NaCl浓度增加,叶绿素a、叶绿素b和总叶绿素含量呈先上升后下降的变化趋势,而叶绿素a/b比值无明显变化;随着盐浓度的增长杂交酸模97-1细胞膜透性增大,丙二醛含量升高;游离脯氨酸及可溶性糖含量与NaCl浓度呈正相关.%The seed germination and sapling physiological index of Rumex 97-1 cultured in vitro under different salt stress were studied. The results showed that there was a very significant negative correlation between seed germination rate and NaCl concentration, the critical value of salt tolerance for seed germination was 0.93％, The seed germination was below 10％ when NaCl concentration was 1.6％～1.8％, the content of chlorophyll a, chlorophyll b and total chlorophyll presented a first rise and then declining trend with increase of NaCl concentration, but the chlorophyll a-chlorophyll b ratio had no obvious change, the membrane permeability and MDA content increased with increase of NaCl concentration and there was a positive correlation between NaCl concentration and free praline, soluble sugar content.
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