Sequence related amplified polymorphism (SRAP) marker was applied to analyze genetic diversity and relationship of 30 tea plant germplasms in Sichuan to provide references for evaluation and identification of tea plant germplasms, screening of core germplasms, genetic improvement, resource protection and utilization. The results showed that eleven pairs of SRAP primers produced 2 744 amplified fragments. Of which 2474 were polymorphic. The polymorphism information index ranged from 0. 22 to 0. 36 with an average of 0. 30. The Nei' s gene diversity index was 0. 342 1~0. 545 5 with an average of 0. 4227. The average Shannon's information index was 0. 271 1, ranging from 0. 204 3 to 0. 371 0. These findings revealed that the germplasms had a great deal of genetic diversity. The dendrogram showed distinct genetic differentiation among germplasms. The genetic similarity coefficient varied from 0. 583 to 0. 919. The UPGMA cluster analysis showed that 30 tea plant germplasms could be clustered into A and B groups when the genetic similarity coefficient was given as 0. 66.%为给四川省茶树种质资源评价与鉴定、核心种质资源筛选、遗传改良、资源保护与利用等提供参考,利用SRAP标记对30份四川茶树种质资源进行遗传多样性与亲缘关系分析.结果显示:11对SRAP引物共扩增出2744条带,其中,2474条为多态性条带,占90.16%;多态性信息指数为0.22~0.36,平均0.30;基因多样性指数为0.342 1~0.5455,平均0.422 7;Shannon信息指数为0.204 3~0.371 0,平均0.271 1.表明,30份茶树资源的遗传多样性程度较高.聚类分析表明,供试材料遗传差异较大,遗传相似系数为0.583~0.919;在遗传相似系数为0.66时,可将30份茶树资源划分为A、B两大类.
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