水产养殖用地衣芽孢杆菌的分离鉴定与其扩增培养基筛选

摘要

为分离筛选适合水产养殖用的地衣芽孢杆菌，并探明不同培养基对其扩增效果，采用微生物学与分子生物学相结合的方法对养殖池塘淤泥中的细菌进行研究。结果表明：分离得到2株抑菌作用最强的菌株DSY002-2011和 DSY003-2011，其中，DSY002-2011菌株为地衣芽孢杆菌。由豆粕与红糖、玉米粉、大米粉和麸皮等分别构成的4种生产型培养基均能将DSY002-2011菌株的细菌数扩增至200亿个以上，其中，以红糖20 g/L＋豆粕80 g/L的组合最优，其细菌扩增培养浓度可达39×109 cfu/mL。%The combined methods of microbiology and molecular biology were employed to isolate and screen the suitable B.licheniformis for aquaculture and explore the amplification effects of different culture media.Results:Two strains DSY002-2011 and DSY003-2011 with the strongest bacteriostatic action were isolated,in which DSY002-2011 was B.licheniformis,the bacterial count could be up to more than 20 billion in four production media composed of bean pulp,brown sugar,corn flour,rice meal and bran.The combination of browning sugar 20 g/L and bean pulp 80 g/L was of the best effects,the multiplication culture concentration of bacteria was up to 39 ×109 cfu/mL.