The genetic diversities of 83 early-maturing litchi(variety) plants were examined by using ISSR markers in this study. Ten primers gave reproducible, polymorphic DNA amplification patterns with a total of 128 amplified bands. The percentage of polymorphic band was 83. 59%,which showed that the early-maturing seedling litchi resources in southwest Guangxi had abundant genetic diversity. The genetic similarity ranged from 0. 64 to 0. 95 based on DICE. A dendrogram was constructed by UPGMA method,and the early-maturing litchi(variety)plants were distinguished from cultivar litchi at a similarity coefficient criterion of 0. 75. No significant correlation between the dendrogram and geographical distance was revealed among the resources.%利用ISSR标记技术对83份早熟荔枝(品种)单株遗传多样性进行分析.筛选出多态性高的10条ISSR引物,共扩增出128条DNA条带,其中多态性带107条,多态性百分率为83.59%,表明桂西南早熟荔枝实生资源遗传多样性较丰富;用NTSYS软件计算出这83份材料的DICE相似系数在0.64～0.95之间,遗传亲缘关系较近;用UPGMA方法构建分子树状图,在相似系数0.75时,可将栽培品种与桂西南早熟实生单株区分开.各地区资源混杂聚类在一起,不能按地区单独聚类.
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