新型带吻合口动脉夹在急性全脑缺血再灌注模型中的应用

摘要

目的评估新型带吻合口动脉夹在急性全脑缺血再灌注模型中的应用价值。方法健康 SD 大鼠35只，随机分为3组：正常组（n=5）：只暴露血管不夹闭；实验组（n=15）：使用新型的带吻合口微型动脉夹进行夹闭；对照组（n=15）：使用传统的微型动脉夹进行夹闭。采用颈总动脉（CCA）阻断法建立全脑缺血再灌注模型，分离暴露双侧颈总动脉后予以微型动脉夹夹闭，进入缺血期，夹闭20min 后移去微型动脉夹去除双侧颈总动脉的夹闭，进入灌注期。实验结束后处死大鼠取全脑大体标本。观察大鼠达到全脑缺血所需的时间、脑组织大体标本、缺血过程中动脉夹的夹闭状况。结果正常组大鼠脑组织大体标本皮层颜色较红润、浅表血管丰富；实验组大鼠脑组织皮层颜色苍白、表面血管塌陷，血流大量少，其中部分出现血液无复流的现象；对照组大鼠脑组织皮层颜色微红、表面血管部分塌陷，血流减少。实验组动脉夹在缺血过程中仅3例出现松动，而对照组动脉夹在缺血过程中有6例出现松动、5例出现滑脱。达到全脑缺血所需的时间实验组较对照组更快。结论新型带吻合口动脉夹可以有效的对血管进行夹闭，使缺血更加彻底，并且在夹闭的过程中有较高的稳定性，可以有效的避免动脉夹的松动、滑脱等现象。%　　Objective To evaluate the application value of new anastomotic artery clamp in acute cerebral ischemia reperfusion model. Methods 35 healthy SD rats, were randomly divided into 3 groups: normal control group (n=5): only exposed vascular clipping; the experimental group (n=15): use the micro artery anastomosis clip for clipping; the control group (n=15): Micro artery using traditional clip for clipping. The common carotid artery (CCA) occlusion reperfusion model of global cerebral ischemia, separate and expose the bilateral common carotid artery after be micro-artery clamp, into the ischemic period, clipping after 20 minutes remove micro artery occlusion clip removal of bilateral carotid arteries, into the reperfusion period. At the end of the experiment, the rats were killed and the whole brain specimen. Time, the brain tissue of cerebral ischemia rats were observed to achieve desired artery specimens, ischemia during the clamping closing conditions. Results In normal group rats brain tissue specimens of cortical color is rosy, superficial vascular rich;brain tissue of the experimental group rat cortical pale, surface of vascular collapse, flow a lot less, part of no-reflow phenomenon of brain blood; the control group rat cortical reddish surface vessels, the partial collapse, reduction of blood flow. The experimental group artery clamp during ischemia occurred in only 3 case of loosening, while the control group, arterial clip during ischemia occurred in 6 cases, 5 cases appeared loose spondylolisthesis. Time of the experimental group reached complete cerebral ischemia required faster than that of the control group. Conclusion The model with anastomotic artery clamp can effectively clamp on the ischemic vascular, more thoroughly, and high stability in the process of clipping, can effectively avoid the phenomenon of artery clamp loosening, dislocation.