Cu2+对两种海洋微藻生长和叶绿素荧光特性的影响

摘要

The changes of the fluorescence parameters of Dicrateria zhanjiangensis and Isochrysis galbana MACC/H59 exposed to Cu2+ were investigated by means of the chlorophyll fluorescence technology(Water-PAM) ,and the growth of these two species of marine microal-gae was observed. The results showed that all fluorescence parameters ( Fv/Fm ,Fv/F0,Yield and ETR) of Dicrateria zhanjiangensis and Isochrysis galbana MACC/H59 decreased under Cu2+ stress. The growth of cell density for Dicrateria zhanjiangensis and Isochrysis galbana MACC/H59 decreased under Cu2+ stress,and it can be expressed as Dicrateria zhanjiangensis > Isochrysis galbana MACC/H59 in the 24h while it can be expressed as Isochrysis galbana MACC/H59> Dicrateria zhanjiangensis during 48h to 96h. The toxicity of Dicrateria zhanjiangensis and Isochrysis galbana MACC/H59 increased with the increase of exposure time.%运用水样叶绿素荧光仪(WATER-PAM)研究Cu2+胁迫下,湛江叉鞭金藻(Dicrateria zhanjiangensis)和球等鞭金藻3011(Isochrysis galbana MACC/H59)叶绿素荧光特性的变化,同时监测微藻的生长情况.结果就叶绿素荧光参数而言,湛江叉鞭金藻和球等鞭金藻3011的叶绿素荧光参数Fv/Fm,Fv/F0,Yield和ETR均随着Cu2+浓度的增大而明显降低；就生长情况而言,随着Cu2+浓度的增大,两种海洋微藻细胞密度的增长明显变缓.在24 h时Cu2+对湛江叉鞭金藻的毒性大于球等鞭金藻3011,48～96 h时Cu2+对球等鞭金藻3011的毒性大于湛江叉鞭金藻,Cu2+的毒性随着胁迫时间的延长而逐步增大.