【目的】获得耐受高铵浓度的优良养殖小球藻（Chlorellavulgaris ）品系，可以有效防止其在养殖过程中被轮虫，纤毛虫等污染生物吞食。【方法】首先对小球藻进行紫外线诱变，然后用高浓度 NH4 HCO3作为筛选压力对其进行筛选。【结果】筛选到具有生长优势的小球藻突变株 B4和 C6，培养12 d，在 NH4 HCO3浓度为800 mg／L、1000 mg／L、1200 mg／L条件下，B4和C6的生物量比出发藻株分别提高41．18％和9．54％，32．64％和29．41％，37．74％和22．85％；对比出发藻株，B4和C6的最大光能转化效率有显著提高。【结论】突变株 B4和C6在高铵培养条件下更能显示生长优势，且它们的生长优势可能来自光合效率的提高。%Objective]Breeding the new strains of Chlorella vulgaris that have a tolerance to high concentration of ammonium can efficiently avoid being eaten by the contamination animals such as rotifer,ciliate and so on during cultivation.[Methods]The original strain of Chlorella vulgaris was first mutated by exposing to UV light and then was screened by the high concen-tration of ammonium in media.[Results]Two mutants,B4 and C6,were acquired with better growth rate than the original one.The biomass of strains B4 and C6 after being cultivated for 12 d increased 41.18% and 9.54%,32.64% and 29.41%,37.74% and 22.85%,respectively, under different ammonium concentration,which was 800 mg/L,1 000 mg/L,and 1 200 mg/L. The Fv/Fm results also proved a significant improvement on their maximum light conversion efficiency.[Conclusion]The mutants B4 and C6 showed growing advantages under higher am-monium concentration,which may be gained from the enhancement of photosynthesis efficien-cy.
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