[目的]探讨广西斜阳岛附近海域海绵共附生细菌的多样性,并筛选对甘蔗鞭黑粉菌有抑制作用的活性物质,为发现潜在的细菌新物种及开发农用生防菌肥奠定基础.[方法]采用稀释涂布法,通过6种复合营养分离培养基,从海绵Pseudoceratina sp.中分离可培养的共附生细菌.采取细菌形态学观察去除冗余,通过PCR扩增菌株的16S rRNA基因序列,并通过构建系统发育树分析物种多样性.采用牛津杯法进行抑制甘蔗鞭黑粉菌活性筛选实验.[结果]从海绵Pseudoceratina sp.中共分离到可培养细菌54株,经16S rRNA基因序列对比后,获得24株细菌,隶属于13科14属.其中,菌株BGMRC 2118与已发表有效菌株的16S rRNA基因序列的最高相似率为96.46％,可能为潜在新种.抑菌筛选实验结果显示,有8株细菌的发酵粗提物对甘蔗鞭黑粉菌具有很强的抑菌活性.[结论]广西斜阳岛附近海域中海绵共附生细菌具有较高的物种多样性,蕴藏着丰富的新物种资源,富含生物活性菌株.%[Objective]The purpose of this study is to investigate the diversity of sponge-derived bacteria of Pseudoceratina sp.near the sea area of Xieyang Island in Guangxi and to screen for the active substances that inhibit the growth of Sporisorium scitamineam and lay the foundation for the discovery of potential bacterial new species and the development of agricultural biochemical fertilizer.[Methods]Using dilution coating method the sponge-derived bacteria were isolated from Pseudoceratina sp.through six different isolation media.The bacterial morphological observation was used to remove the redundancy.The 16S rRNA gene sequence of the strain was amplified by PCR,and the species diversity was analyzed by constructing a phylogenetic tree.The Oxford Cup method was used to the screening experiment of inhibiting the activity of Sporisorium scitamineam.[Results]54 strains of cultivable bacteria were isolated from Pseudoceratina sp.,and after 16S rRNA gene sequence comparison,24 strains of bacteria were obtained,belonging to 13 families and 14 genera.Among them,the highest similarity rate of strain BGMRC 2118 to the 16S rRNA gene sequence of the published effective strain was 96.46％,which might be a potential new species.Anti-bacterial screening experiments showed that the crude fermentation extracts of 8 strains had a strong inhibition effect against S.scitamineum.[Conclusion]The sponge-derived bacteria of Pseudoceratina sp.near the sea area of Xieyang Island in Guangxi had high genetic diversity,rich in new species resources and rich in bioactive strains.
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