目的 探讨rhBMP-2/20%-β-TCP/SF复合物的生物相容性及其作为骨替代材料的可行性.方法 将12只健康雄性新西兰兔随机分为实验组和对照组,每组6只,按Thomas方法,在其左侧股骨内侧髁作9 mm×4 mm×5 mm的骨缺损区模型,然后将rhBMP-2/20%-β-TCP/SF复合物材料植入实验组,对照组植入自体骨碎片.分别于术后4周、8周、12周各处死2只动物模型进行大体观察,以及进行X线、人工骨降解情况及早期骨组织学切片分析.结果 术后12周实验组植入材料基本完全溶解,并形成典型的骨小梁及髓腔结构,成骨面积为50.45%,大于对照组的42.17%.结论 rhBMP-2/20%-β-TCP/SF复合物具有良好的生物相容性,能有效促进骨缺损的修复愈合,有望成为修补股骨缺损的人工骨移植材料.%Objective To explore the biocompatibility of rhBMP-2/20% - β-TCP/SF composite and the feasibility of applying the composite to bone implantation. Methods Twelve rabbits were divided into two groups in the study: experimental group( n = 6 ) and control group( n = 6 ). Bone defects of 9 mm × 5 mm × 4 mm were made at the entocondyle of left side of thighbone in rabbits. rhBMP-2/20%-β-TCP/SF composite was used as the implant in the experimental group, while autologous bone piece was used in the control group. Two animals were killed at 4 weeks,8 weeks and 12 weeks after operation for gross observation, X-ray observation, artificial bone degradation and early histological manifestations were done respectively. Results After 12 weeks,rhBMP-2/20%-β-TCP/SF composite was almost degraded in the experimental group; The trabecular bone and canal structure were also formed in experimental group. The osteogenesis area of experimental group was larger than that of control group( 50.45% vs 42.17% ). Conclusion rhBMP-2/20%-β-TCP/SF composite is a kind of artificial bone substitute material with good biocompatibility,which can effectively accelerate the healing of bone defects.
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