Objective To explore the expression of p28GANK protein in hepatocellular carcinoma (HCC) tissues and its clinical significance .Methods Sixty specimens of HCC tissues and matched para-carcinoma tissues ( 32 of which contained hepatocirrhosis ) ,30 specimens of normal liver tissues were collected .Immunohistochemistry was used to detect the protein expression of p 28GANK in HCC tissues,para-carcinoma tissues,hepatocirrhosis tissues and normal liver tissues .The relationship between the expression level of p 28GANK protein and clonicopathological features of patients with HCC was analyzed .Results The positive expression rates of p 28 GANK protein in HCC tissues,para-carcinoma tissues,hepatocirrhosis tissues and normal liver tissues were 71.7%,10.0%,12.5%,6.7%,respectively. The positive expression rate of p28GANK protein in HCC tissues was significantly higher than that in the other tissues (P<0.05).In the patients with HCC,the positive expression rates of p28GANK protein in the patients with poorly differentiated type (Class Ⅲ-Ⅳ),with lymphatic/distant metastasis ,and with TNM stageⅢ+Ⅳwere higher than those in the patients with well differentiated type ( ClassⅠ-Ⅱ) , without lymphatic/distant metastasis,and with TNM stage Ⅰ+Ⅱ respectively(P<0.05).Conclusion The expression of p28GANK protein might be associated with tumorigenesis and progress of HCC .And p28 GANK may become a molecular marker for the diagnosis of HCC.%目的：探讨p28GANK蛋白在肝细胞性肝癌（HCC）组织中的表达及其临床意义。方法收集HCC组织及其相应癌旁组织60例（其中32例伴有肝硬化组织）、正常肝组织30例，采用免疫组化法检测HCC、癌旁组织、肝硬化组织、正常肝组织组织中p28GANK蛋白的表达，分析p28GANK蛋白表达水平与HCC患者临床病理特征关系。结果 p28GANK蛋白在HCC、癌旁肝组织、肝硬化组织性、正常肝组织阳性表达率分别为71．7％、10．0％、12．5％、6．7％，HCC中p28GANK蛋白的阳性表达率高于其他组织（P＜0．05）。 HCC患者中，低分化（Ⅲ～Ⅳ级）、淋巴结和（或）远处转移及TNM分期Ⅲ＋Ⅳ者p28GANK蛋白表达阳性率分别高于高分化（Ⅰ～Ⅱ级）、无淋巴结和（或）远处转移、TNM分期Ⅰ＋Ⅱ者（P＜0．05）。结论 p28GANK蛋白表达可能与HCC的发生发展有关，p28GANK可作为HCC诊断的分子标志物。
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