脱氮硫杆菌利用不同硫源去除地下水硝酸盐的实验研究

摘要

从斛兵塘底土壤中分离得到一株自养反硝化菌。该菌株经过生理生化实验，初步判定为脱氮硫杆菌。该菌株可以分别利用黄铁矿、硫代硫酸钠、硫单质作电子供体，进行反硝化代谢。实验表明，在黄铁矿存在下该菌种对硝酸盐氮去除率最高，同时也有较商的硫酸根生成率。为了去除硫酸根新污染，尝试在培养基中加入石灰石。经实验证明．在培养基中加入石灰石几乎不影响反硝化代谢，并且有效的去除硫酸根离子，达到去除硝酸盐的目的。%A strain of Thiobacillus was isolated from the soil of the bottom of the Hubing Pool. Based on the analysis of physiological and biochemical measurements, this stain was identified with Thiobacillus denitrificans. The stain was employed to reduce Nitrate nitrogen by using of pyrite, thiosulfate and brimstone as electron donor. Experiments show that, the T.D get the highest nitrate nitrogen removal rate by using of pyrite, and also higher sulfate formation rate. In order to remove the sulfate pollution, try to add limestone in the pyrite medium, The pyrite medium by adding limestone make the nitrate nitrogen removal rate Almost no decreased, and effective removal of sulfate produced from denitrification, and ultimately achieve the purpose of remove the nitrate.