黄芪对急性肺损伤模型大鼠肺组织水通道蛋白-1和水通道蛋白-5表达的影响

摘要

目的 探讨补气中药黄芪对急性肺损伤模型大鼠肺组织水通道蛋白-1(AQP-1)和水通道蛋白-5(AQP-5)表达的影响.方法 将30只雄性SD大鼠随机分为正常对照组、模型组、中药组各10只,予以每ml相当于0.25 g黄芪生药的煎剂干预后,使用凝胶电泳测条带积分光密度值来检测确定肺组织AQP-1和AQP-5的表达情况.结果 中药组大鼠肺组织AQP-1的积分光密度值为(0.572±0.084),模型组大鼠肺组织AQP-1的积分光密度值为(0.278±0.068),中药组高于模型组(P＜0.01).中药组大鼠肺组织AQP-5的积分光密度值为(0.812±0.084),模型组大鼠肺组织AQP-5的积分光密度值为(0.525±0.045),中药组高于模型组(P＜0.05).中药组与正常对照组无明显差异(P＞0.05).结论 黄芪可能是通过促进肺组织AQP-1、AQP-5的表达,从而起到对模型大鼠急性肺损伤的防治作用.%Objective To investigate the effect of supplementing qi herbs astragalus mongholicus (huangqi) on AQP-1 and AQP-5 expression of lung tissue of ALI model rat induced by LPS. Methods 30 male Sprague Dawley rats were randomly divided into normal control group, model group, herbs group,each 10. Test AQP-1mRNA and AQP-5mRNA expression of lung tissue, after the supplementing qi herbs used. Results AQP-1mRNA IOD of herbs group (0. 572±0. 084), AQP-1mRNA IOD of model group (0. 278±0. 068), herbs group higher than model group (P<0. 01); AQP-5mRNA IOD of herbs group (0.812±0. 084), AQP-5mRNA IOD of model group (0. 525±0. 045), herbs group higher than model group (P<0. 05); There were no statistical significance between herbs group and normal control group (P>0. 05). Conclusion Supplementing qi herbs astragalus mongholicus prevent and treat the ALI probably by promoting the AQP-1mRNA and AQP-5mRNA expression of lung tissue.