苦参碱对合轴马拉色菌麦角固醇生物合成的抑制作用

摘要

Objective To test the Malassezia sympodialis ergosterol concentrations by HPLC to discuss mechanism on matrine inhibition of malassezia. Methods The ergosterol concentrations of Malassezia sympodialis was checked after matrine or ketoconazole affected by High Performance Liquid Chromatography (HPLC). Results Two groups can both inhibite ergosterol(P = 0. 65>0. 05),and have no significance. Two groups had abvious differences of malassezia inhibitions on clinical strains when compared with standard strains, two treatment groups both had stronger inhibition in standard groups than clinical groups ( P = 0. 02 < 0. 05). Conclusion Matrine has inhibitions of ergosterol of Malassezia sympodialis as well as ketoconazole. Its inhibition mechanism of Malassezia sympodialis might have relationship of inhibition of ergosterol biosynthesis.%目的：观察苦参碱对合轴马拉色菌麦角固醇生物合成的影响，探讨苦参碱抑菌机理。方法本研究分为苦参碱组、酮康唑组及空白组，分别通过高效液相色谱法检测8天后三组培养基中合轴马拉色菌的麦角固醇含量，最后采用 SPSS 17.0对数据进行分析处理。结果苦参碱组、酮康唑组与空白组相比，差异有统计学意义(P<0.01)，说明两组均有抑制麦角固醇合成的作用，苦参碱组与酮康唑组两者之间差异无统计学意义(P =0.65>0.05)，说明两组抑制麦角固醇合成的作用相当。体外抑菌试验中两组药物对于合轴马拉色菌临床株和标准株的抑制强弱有明显差异(P<0.05)，两组药物对于标准菌株的抑菌效果均明显强于临床菌株。结论苦参碱可以抑制合轴马拉色菌麦角固醇合成，作用与酮康唑相当，考虑其抑菌机制可能与破坏麦角固醇合成有关。