Pseudomonas putida MnB1氧化分解菱锰矿的实验研究

摘要

Rhodochrosite (MnCO3) is a solid Mn(Ⅱ) origin mineral and its oxidation into Mn oxides is a very common phenomenon. Although microbially mediated oxidation of Mn(II) to Mn oxides have been demonstrated in previous studies, the mechanisms of bacteria how to dissolve and oxidize using a solid Mn(II) origin are poorly understood. In this study, we examined the role of Pseudomonas putida MnB1 cell in enhancing dissolution and oxidation of rhodochrosite from Wuzhou, Guangxi province. By using Field Emission Scanning Electron Microscopy (FESEM), Scanning Transmission X-ray Microscopy imaging analysis (STXM) as well as other techniques, the changes of the mineral surfaces and the distribution of manganese in cells were identified. The result show that bacteria significantly promote the dissolution of the rhodochrosite. Moreover, the contribution of bacteria to the oxidation of rhodochrosite was discussed. The results will enrich the research of secondary manganese deposits.%自然界中，菱锰矿氧化形成锰的氧化物矿物是非常普遍的现象，在菱锰矿被氧化分解发生物相转变的过程中，碳酸盐溶解和锰的氧化往往同时发生，微生物可能起着催化作用。选取锰氧化模式菌株Pseudomonas putida MnB1和广西梧州菱锰矿，通过菱锰矿在该细菌作用下发生转变的实验，利用场发射扫描电镜、扫描透射X射线显微成像等分析方法，研究了矿石表面形貌变化以及锰元素在细胞上的分布特征。结果表明细菌显著促进的菱锰矿的溶解，在此基础上，进一步探讨了细菌在菱锰矿氧化过程中的贡献，本实验结果丰富了次生锰矿床的微生物成因研究。