猪繁殖与呼吸综合征病毒(PRRSV)欧洲株和美洲株的理化特性及致病性比较

摘要

对分离自福建的3株美洲型猪繁殖与呼吸综合征病毒(PRRSV)与2株欧洲型PRRSV进行了细胞致病性、动物致病性及理化特性的比较试验,结果显示PRRSV分离株均能在Marc-145细胞上增殖并产生细胞病变,但在PK-15、Vero、CEF、MDEF中盲传3代均不能产生细胞病变.理化性质试验表明美洲株与欧洲株PRRSV对酸、pH=3、氯仿、胰酶的敏感性一致；当pH=9时,PRRSV欧洲株比美洲株具有更强的抵抗力；不同的PRRSV毒株对温度的敏感性差异较大,同一型的病毒对温度的敏感性也不同.动物致病性试验结果表明欧洲型PRRSV-FJ0602株为弱毒株,对保育猪不具有致病性,而美洲型PRRSV-FJ0604株致病力强.%The Physicochemical Characteristics and Pathogenicity of five PRRSV strains including two European types PRRSV and three North American types PRRSV were studied, which were isolated from pig farms in Fujian province. The results showed that all the five PRRSV strains could induce regular cytopathogenic effects (CPE) on Marc-145 cells, but CPE was not appeared on PK-15, Vero, CEF or MDEF cells after three times of blind passages. The five PRRSV strains showed the same sensitive to acid, pH value 3, chloroform and trypsin, but different to the temperature. Compared with the North American types PRRSV, the European types PRRSV were more resistant to pH value 9. It was confirmed that the European type PRRSV-FJ0602 has a low pathogenicity to pigs but the North American type PRRSV-FJ0604 has a high pathogenicity to pigs according to the animal experiment.