粳稻云引受稻瘟病菌诱导表达基因的荧光定量分析

摘要

In early stage, we analysed the gene expression profile of broad-spectrum rice blast resistance japonica variety Yunyin induced by Mangnaporthe oryzae, and constructed the gene co-expression network based on the dynamic samples. Ten important genes based on this network were chosen in this study, and the expression pattern of 10 genes for japonica rice Yunyin inoculated with Mangnaporthe grisea was analyzed. The highest expression stages of the 10 genes were different, of which 8 genes reached the highest expression stage at 36 hours, and 3 genes encoding transcription factors reached the highest expression stage at 24 hours. Meanwhile, some of the 10 genes expression were up-regulated at first, and then inhibited. Others were inhibited at first, then increased, and declined at last. This work will help us further to define the role of japonica rice Yunyin in disease resistance.%前期通过对广谱稻瘟病抗性品种粳稻云引接种稻瘟病菌后的基因表达谱分析,构建随时间变化的动态样本基因调控网络.在此基础上,选取该调控网络中10个比较重要的基因,采用Real-Time PCR的方法对其在接种稻瘟病菌后的时空表达进行相对定量分析,得到这些基因在稻瘟病菌诱导后在云引中的时空表达模式.10个基因均受到稻瘟病菌的诱导,但达到表达量峰值的时间各异,有8个基因在36 h内表达量达到峰值,3个编码转录因子的基因表达量在24 h内达到峰值；部分基因表达呈先上升,后抑制,另一些基因的表达则先抑制,后上升,再下降.该研究为进一步确定这些基因在云引抗病性调控中的作用奠定基础.