云引稻瘟病抗性基因型的初步分析及其近等基因系构建

摘要

The phenotype of broad-spectrum rice blast resistance variety Yunyin was analyzed and identified by inoculated with rice blast fungi RB01-RB22 of known avirulence genes as pathogen in vitro. The results showed that Yunyin was resistant to all rice blast fungi except RB17 and RB20. Analyzing the widely used 24 resistance genes in production at present, the results showed that Yunyin may contain new blast resistance genes besides these genes, and there were able to be applied for rice breeding and production. In addition, in this study, we constructed near-isogenic lines with LTH as genetic background and contained blast-resistant gene to blast isolate SiChuan 43 by successive backcrossing combining marker-assisted selection with artificial inoculation. These works would be beneficial to the genetics study and application of this blast-resistance gene.%利用水稻品种稻瘟病抗性基因型鉴定菌株RB01～RB22,对云引苗期叶片进行离体接种,结果表明云引除对RB 17和RB20感病外,对其他菌株均表现抗病,通过分析RB01～RB22对目前生产上广泛应用的24个稻瘟病抗性基因的抗性鉴定结果表明,这些基因的反应型均与云引不同,即可能含有除上述抗病基因之外的新的稻瘟病抗原.此外,通过连续回交、分子标记辅助选择和人工注射接菌相结合的方法,构建了以普感稻瘟病品种丽江新团黑谷(LTH)为遗传背景,含有抗稻瘟病菌株“四川-43”基因的近等基因系,为该抗病基因的遗传学研究和应用构建了良好的载体.