[目的]杨生褐盘二孢的单芽管专化型和多芽管专化型分别引起白杨组和黑杨组(包括青杨组)杨树的褐斑病,其分化机制有待阐明.[方法]本文在专化性互作体系建立的基础上,利用荧光染色标记研究了病菌孢子在专化性寄主侵染过程中的萌发发育情况,并通过RT-qPCR分析了黑杨寄主抗病相关基因在应答两专化型侵染过程中的不同表达.[结果]结果发现多芽管分生孢子在黑杨寄主上能完成发育并成功侵染,单芽管分生孢子也能成功萌发和发育,但不能侵入黑杨寄主;黑杨抗性基因在两专化型侵染发育过程中均能被诱导表达,但在表达时间和表达量上,除基因WRKY89的表达较为一致外,病程相关基因PR5、PR10、NPR1和LAR3在二者间的表达存在差异.[结论]研究结果初步表明病原专化型可能是寄主与病原专性互作的表型,这一认识将有助于进一步探明杨树褐斑病病原专化型形成和分化的机制.%[Objective] To study the mechanism of Marssonina brunnea sp.monogermtubi formae speciales (RHBH) infecting Populus Section Leuce and M.brunnea sp.multigermtubi formae speciales (RHHB) infecting Populus Section Aigeiros.[Method] Based on pathogen-host interaction system,the pathogen's spore germination,growth and infection process on poplar leaves were investigated by using fluorescence microscopy strain method and the expression of five pathogen-relative genes in hosts was also analyzed by RT-qPCR technique.[Result] The results showed that the spores of RHHB were able to grow and successfully infect the hosts of Populus Section Aigeiros,the spores of RHBH were also able to grow but failed to infect the hosts of Populus Section Aigeiros.The pathogen-resistant genes of Populus Section Aigeiros could be induced and express during the infection process of the two formae speciales.The gene express of WRIKY89 followed a similar expression trend,while the other four genes (PRS,PR10,NPR1 and LAR3) followed different expression patterns in time and quantity for the two formae speciales.[Conclusion] The study provides some references for study the mechanisms of the two formae speciales and their differentiation.
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