5-（4-（2-甲氧二氧）苯烯）-2-硫代噻唑-4-酮对雪莲果中酪氨酸酶的抑制作用

摘要

通过分光光度法探究5-（4-（2-甲氧二氧）苯烯）-2-硫代噻唑-4-酮（MBTO）对雪莲果中酪氨酸酶的抑制作用。结果表明：MBTO对雪莲果中酪氨酸酶有良好的抑制作用。单因素试验最佳抑制剂浓度为30.0μmol/L、最佳作用时间为210s、最佳作用温度为30℃。通过三因素三水平正交试验,分析得最佳反应条件为：pH7.5、抑制剂MBTO浓度30.0μmol/L、作用时间330s、作用温度30℃,可达到最佳抑制效果,在此条件下MBTO对雪莲果中酪氨酸酶的抑制率达84.24%。%The inhibitory effect of（Z）-5-（4-（2-methoxyethoxy）benzylidene）-2-thioxothiazolidin-4-one（MBTO） on tyrosinase in yacon was studied by spectrometrically.The results showed that MBTO had excellent inhibitory effect on tyrosinase in yacon.The optimal inhibitory concentration,time and temperature,as determined by one-factor-at-a-time design,were 30μmol/L,210 s,and 30 ℃,respectively.The results of 3-variable,3-level orthogonal array experiments showed that the best inhibition results were obtained by treatment with 30.0μmol/L MBTO at pH 7.5 and 30 ℃ for 330 s,yielding an inhibitory rate as high as 84.24%.