UPLC-DAD/ESI-TOF-MS鉴定黑脉羊肚菌多酚化合物

摘要

以黑脉羊肚菌为原料,分离提取羊肚菌多酚(游离酚和结合酚),对提取物中酚类物质的含量及抗氧化活性进行研究,并采用超高效液相色谱-二极管阵列检测器/电喷雾飞行时间质谱对黑脉羊肚菌游离酚和结合酚的组分进行鉴定.结果表明,黑脉羊肚菌多酚主要为游离酚,且游离酚的DPPH自由基清除率、还原力及氧自由基吸收能力都显著强于结合酚(P＜0.05),而两者清除ABTS+·能力相当.从黑脉羊肚菌多酚中鉴定出15种组分,分别是没食子酸、焦性没食子酸、原儿茶酸、对羟基苯甲酸、儿茶素、咖啡酸、绿原酸、荭草素、芦丁、金丝桃苷、白藜芦醇、木犀草素、槲皮素、肉桂酸、阿魏酸,游离酚提取物有15种组分,结合酚提取物有14种组分.%Free and bound phenolics from Morchella angusticeps Peck were separately extracted and analyzed by ultra performance liquid chromatography-diode array detector/electrospray ionization-time of fight-mass spectrometry (UPLC-DAD/ESI-TOF-MS).The antioxidant activities of the phenolic extracts were assessed.The results showed that phenolics were present mainly in the free form in Morchella angusticeps Peck.The DPPH (1,1-dipheny1-2-picryl-hydrazyl) scavenging capacity,reducing power and oxygen radical absorption capacity (ORAC) value of free phenolics from Morchella angusticeps Peck were significantly higher than those of bound ones (P ＜ 0.05),but no significant difference in ABTS+.(2,2'-amino-di (3-ethyl-benzothiazoline sulphonic acid-6) ammonium salt) scavenging capacity existed between free and bound phenolics.A total of 15 polyphenols in Morchella angusticeps Peck,including gallic acid,pyrogallic acid,protocatechuic acid,p-hydroxybenzoic acid,catechin,caffeic acid,chlorogenic acid,orientin,rutin,hyperoside,resveratrol,luteolin,quercetin,cinnamic acid and ferulic acid,were identified.We identified 15 and 14 components in the free and bound phenolic extracts,respectively.