免疫学方法在食源性副溶血性弧菌检测中的应用研究进展

摘要

The bacterial pathogen Vibrio parahaemolyticus (VP) can pollute a variety of foods,especially seafoods.Foodbome VP has surged as the primary pathogen in food safety incidents in China.Compared to traditional cultural methods and modern molecular techhniques,specific antigen-antibody recognition-based immunological identification and subtyping strategies exhibit superiority in efficiency,specificity,on-field applicability and simplicity of pre-treatment.In this paper,the category and preparation methods of anti-VP antibodies at present are outlined.Furthermore,we review the current applications of immunological methods such as immunomagnetic separation (IMS),enzyme-linked immunosorbent assay (ELISA),immunochromatographic assay (ICA),immunosensor and immunomicroscopy in the detection of foodborne VP,which will provide useful references for rapid detection of VP.%副溶血性弧菌可污染以海产品为主的各类食品,其引发的食品安全事件数量已经跃升至我国食品中毒事件数首位.相对于传统培养法及现代分子检测技术,基于抗原-抗体特异性识别反应的免疫鉴定及分型策略在检测效率、特异性、现场适用性、前处理简便性等诸多方面显现突出优势.本文在解析当前副溶血性弧菌抗体种类及制各方法的基础上,就免疫磁珠分离、酶联免疫吸附测定、免疫层析测试、免疫传感器、免疫荧光显微术等方法在食源性副溶血性弧菌检测中的应用现状进行综述,以期为该病原菌的快速检测提供借鉴和参考.