基于拉曼光谱法测定冷鲜牛肉中肌红蛋白相对含量

摘要

为研究4℃贮藏期间冷鲜牛肉3种化学形式肌红蛋白相对含量变化,对采集的一维拉曼光谱,用2D-shige二维相关分析软件对其光谱数据进行二维相关分析,得出3种化学形式肌红蛋白的相对含量最佳推导公式.结果表明:肌红蛋白、氧合肌红蛋白与高铁肌红蛋白相对含量的最佳推导公式分别为:脱氧肌红蛋白相对含量/％=(-0.305 5x+ 19.570)×100;氧合肌红蛋白相对含量/％=(-2.933 9x+ 154.17)×100;高铁肌红蛋白相对含量/％=(1.574 3x-14.369)×100.3种化学形式的肌红蛋白相对含量的预测效果为高铁肌红蛋白＞脱氧肌红蛋白＞氧合肌红蛋白,其中波数为1 033～1 248 cm-1时,高铁肌红蛋白相对含量的误差值最小为4.76％.研究表明,基于拉曼光谱法无损检测冷鲜牛肉中肌红蛋白相对含量是可行的.%In this study,we examined the changes in the relative amounts of three chemical forms of myoglobin,deoxymyoglobin (DeoxyMb),oxymyoglobin (OxyMb) and metmyoglobin (MetMb),in fresh beef meat stored at 4 ℃.For this purpose,one-dimensional Raman spectral data were acquired and analyzed by two-dimensional correlation analysis using 2D-shige software.The optimal formulas for the relative amounts of three chemical forms of Mb were DeoxyMb/％ =(-0.305 5x + 19.570) × 100;OxyMb/％ =(-2.933 9x + 154.17) × 100;and MetMb/％ =(1.574 3x-14.369) × 100,respectively.The prediction efficiency of these formulas was in the decreasing order:MetMb ＞ DeoxyMb ＞ OxyMb.When the Raman frequency was 1 033-1 248 cm-1,the root mean square error of prediction of MetMb was minimum,only 4.76％.Therefore,it is feasible to determine the relative amount of myoglobin using Raman spectroscopy.