凡纳对虾溶菌酶基因的克隆与性质研究

摘要

凡纳对虾先天免疫系统中的抗菌肽效应分子是发挥抗菌作用的关键分子，其中，溶菌酶分子在对抗细菌的侵染过程中，发挥着重要的清除细菌的作用。以凡纳对虾溶菌酶基因作为研究对象，通过进行基因克隆、氨基酸序列比对、系统进化关系分析以及分子结构的初步预测，推测该溶菌酶基因在凡纳对虾先天免疫系统中发挥着重要的抗菌作用。%　　Antimicrobial peptide effectors played the key and important antibacterial role in the Litopenaeus vannamei innate immunity system. And the lysozyme also played an important part in the clearance of bacteria during the bacteria infection process. In this paper , Litopenaeus vannamei lysozyme gene was the research object. And the gene cloning, the amino acids sequence alignment, the phylogenetic relationship analysis, and the preliminary forecasts for molecular structure were done. We speculated that the lysozyme gene played an important part in the antibacterial function in the Litopenaeus vannamei innate immunity system.