响应面法对牛喉管软骨多糖提取的工艺优化

摘要

In order to study the extraction process of cartilage polysaocharide ,the costal cartilage of bovine was selecIed as materials and the enzymatic processing technology for polysaccharide was studied in this paper. Through Response Surface Methodology, the optimum process parameters were extracted for 9.33 h at pH8.23 and 53.41℃. Under this optimal condition, the extraction rates was 20.05%and the content of polysaccharides was 39.58 %. Through High Performance Liquid Chromatography , the Cartilage polysaccharides was were determined as the similar of chondroitin sulfate.%　　选取哺乳动物牛的喉管软骨为实验材料，用酶法提取多糖并运用响应面法对其提取工艺中温度、pH、搅拌时间进行了优化分析，得到的最佳工艺条件为温度53.41℃、pH8.23连续搅拌酶解9.33 h；此条件下的多糖提取率为20.05%，酸性多糖含量达39.58%。通过高效液相色谱法，初步断定软骨多糖为类硫酸软骨素产品。