以马铃薯克新1号及青薯168为试供材料，采用二因素完全随机设计，在100%、85%、15%、0%光照强度下贮藏90 d，用高效液相色谱法（RPHPLC）测定不同萌芽状态时（萌芽前、萌芽时、萌芽后）块茎中腐胺、亚精胺和精胺的含量。结果表明：不同光照下两个品种萌芽前多胺含量差异均不显著（P>0.05）；萌芽前克新1号块茎中腐胺和亚精胺均高于青薯168，精胺含量较低，但萌发时克新1号精胺增幅（增加约3~10倍）大于青薯168；不同光照下两个品种的腐胺含量都呈先上升后下降的趋势，萌发时大小顺序均为85%光照>100%光照>15%光照>0%光照，各处理差异显著（P0%光照>85%光照>100%光照，各处理差异显著（P15%光照>100%光照>0%光照，亚精胺含量越高萌芽数越多，随了芽的生长亚精胺都有所增加；两个品种萌发时精胺含量大小顺序与腐胺一致，精胺含量越高萌芽越早，萌发后精胺含量大于萌发前，大小顺序为100%光照>85%光照>15%光照>0%光照，精胺含量越高芽越长。%With the Kexin No.1 potato and Qingshu 168 potato original seed as the test for materials , we use two factors completely random design, in 100 %, 85 %,15 %,0 %light illumination store potato 90 d. A highly sensitive and accurate reversed phase high performance liquid chromatographic (RPHPLC) method was developed to determine putrescine, spermidine and spermine of potato tube at different germination status (before sprouting, in the sprouting and after sprouting). The results showed that before sprouting polyamine of two varieties in different storage illumination had no significant difference (P>0.05). Putrescine and spermidine in Kexin No.1 tuber were higher than Qingshu-168 and spermine content was lower, but the content of spermine increase (increased about 3~10 times) larger than Qingshu-168 in the sprouting. Putrescine of two varieties were first increased and then decreased in different storage illumination. Their order of size was 85 %illumination , 100 %illumination, 15 %illumination and 0%illumination, they had significant differences (P<0.01)in the sprouting. The more the putrescine produce, the sooner the buds germinate. After sprouting the putrescine was decreased than before sprouting and their order of size was 15 % illumination, 0 % illumination, 85 %illumination and 100 %illumination, they had significant difference(P<0.01). The more the putrescine produce, the thicker the buds grow. In the budding the spermidine's order of size was 15%illumination, 0%illumination, 85 % illumination and 100 % illumination, the more the putrescine produce, the more the buds grow. The content of spermidine increase with buds grow. The spermine's order of size as putrescine , the more the spermine produce, the sooner the buds germinate. After sprouting, the spermine content greater than before sprouting, their order of size is 100 %illumination, 85 %illumination, 15%illumination and 0%illumination. The more the spermine produce, the longer the buds grow.
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