白灵菇液体发酵工艺优化

摘要

The optimum process conditions of pleurotus nebrodensis liquid fermentation was studied according to the index of mycelia biomass in this paper. Under the conditions of 25℃,160 r/min,eight days,inoculum size 10%and volume 80 mL/250 mL,the optimal composition of the medium:glucose 3.0%,peptone 0.4%,KH2PO4 0.3%, MgSO4 0.2%by single factor experiment and orthogonal experiment. In this condition,the mycelium biomass was 1.27 g/80 mL.%以菌丝体生物量为考查指标，研究了白灵菇（山东寿光720）液体发酵的最佳工艺条件。在培养温度为25℃、摇床转速160 r/min、培养时间为8 d、接种量10%、装液量80 mL/250 mL的条件下，通过单因素试验和正交试验得出最佳发酵培养基配方：葡萄糖3.0%、蛋白胨0.4%、KH2PO40.3%、MgSO40.2%。在此条件下，菌丝体生物量最高可达到1.27 g/80 mL。